Terrence E. Riehl, Ph.D.

Terrence E. Riehl, Ph.D.

Research Assistant Professor

Dr. Riehl joined the laboratory of Dr. William F. Stenson in the Division of Gastroenterology in 1987.  He received his Masters degree at Rutgers University (1974) and his PhD at Ohio University (1981) in Plant Physiology.  He completed Post Doctoral studies at Wake Forest University in the Department of biology, in the area of Developmental Plant Physiology, and in the Department of Chemistry, in the area of micellar chemistry and membrane mimetics.  Dr. Riehl’s current research in the area of intestinal injury and repair is carried out under the guidance of Dr. Stenson.

 

Cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) are the enzymes that convert arachidonic acid to prostaglandins (PG).  Dr. Riehl uses a variety of knockout mouse models to study the role of prostaglandins, most notably PGE2 , in the host response to intestinal injury.  Mouse models of injury have included gamma radiation, DSS, and enteric infection.  We have found that in response to injury COX-2 and PGE2 are crucial in the downstream signaling events leading to the activation of Toll-like receptors, which are an important component of the innate immune system.  We have also found that improvement of injury, as observed histologically, i.e. reduced apoptosis, and by improved clinical scores, can be afforded by such disparate agents as lipopolysaccharide, azoxymethane, and hyaluronic acid.

 

Dr. Riehl also collaborates with other researchers within and outside the Division of Gastroenterology in the interest of learning more about the role of cyclooxygenases and their products in the digestive and other organ systems.

 

 

Selected Publications:

  1. Riehl TE, Newberry RD, Lorenz RG, and Stenson WF.  TNFR1 mediates the radioprotectiveeffects of lipopolysaccharide in the mouse intestine.  Am. J. Physiol. (Gastroent. Liver Physiol.)  286:G166-G173, 2004. 
  2. Murmu N, Jung J, Mukhopadhyay D, Houchen CW, Riehl TE, Stenson WF, Morrison AR, Dieckgraefe BD, and Anant A.   Dynnamic antagonism between RNA-binding Protein CUGBP2 annd cyclooxygenase-2-mediated prostaglandin E2 in radiation damage.  Proc. Natl. Acad. Sci.  101:13873-13878, 2004.
  3. Tessner TG, Muhale F, Riehl TE, Anant S, Stenson WF. Prostaglandin E2 reduce radiation- induced epithelial apoptosis through a mechanism involving AKT activation and BAX  translocation. J. Clin. Invest. 114:1676-1685;2004.
  4. Anant S, Murmu N, Houchen CW, Mukhopadhyay D, Riehl TE, Young SG, Morrison AR,   Stenson WF, Davidson NO. Apobec-1 protects intestine from radiation injury through  posttranscriptional regulation of cyclooxygenase-2  expression. Gastroenterology 127:1139-49;2004.
  5. Ebach DR, Riehl TE, Stenson WF. Opposing effects of tumor necrosis factor receptor 1 and 2 in sepsis due to cecal ligation and puncture.  Shock 23(4):311-318,2005.
  6. Riehl TE, George RJ, Sturmoski MA, May RJ, Dieckgraefe BK, Anant S, Houchen CW.   Azoxymethane protects intestinal stem cells ans reduces crypt epithelial mitosis through a COX-1 dependent mechanism.  Am. J. Physiol. (Gastroent. Liver Physiol.) 291:G1062-G1070;2006.
  7. Westover EJ, Lin X, Riehl TE, Ma L, Stenson WF, Covey DF,  Ostland RE.  Rapid transient absorption and Biliary secretion of enantiomeric cholesterol in hamsters.  J. Lipid Res. 47;2374-2381;2006.
  8. Brown S, Riehl TE, Walker M, Geske M, Doherty J, Stenson WF, Stappenbeck T.  Myd88-dependent positioning of Ptgs2-expressing stromal cells maintains colonic epithelial proliferation during injury.   J. Clin. Invest. 117:258;2007.
  9. May R, Riehl TE, Hunt C, Sureban SM, Anant S, Houchen CW. Identification of a novel putative gastrointestinal stem cell and adenoma stem cell marker, doublecortin and CaM kinase-like-1, following radiation injury and in adenomatous polyposis coli/multiple intestinal neoplasia mice.  Stem Cells.  2008 Mar;26(3):630-637.    
  10. Zheng L, Riehl T, Stenson WF. Regulation of Colonic Epithelial Repair in Mice by Toll-like Receptors and Hyaluronic Acid. Gastroenterology. 2009.  In Press.
  11. May R, Sureban SM, Hoang N, Riehl TE, Lightfoot STA, Ramanujam R, Wyche JH, Anant S, Houchen CW.  DCAMKL-1 and LGR5 Mark Qulescent and Cycling Intestinal Stem Cells Respectively. Stem Cells. 2009. In press.

 

Division of Gastroenterology
Department of Medicine
Washington University School of Medicine